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New England Biolabs
ndei digested pgem t easy ptyaa02 ![]() Ndei Digested Pgem T Easy Ptyaa02, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/ndei+digested+pgem+t+easy+ptyaa02/bio_rxiv__64898__2026__01__29__700732-192-18-24?v=New+England+Biolabs Average 99 stars, based on 1 article reviews
ndei digested pgem t easy ptyaa02 - by Bioz Stars,
2026-07
99/100 stars
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Journal: bioRxiv
Article Title: Periodontal pathogen-derived extracellular vesicles promote EGFR-dependent malignant traits in human pancreatic cancer cells
doi: 10.64898/2026.01.29.700732
Figure Lengend Snippet: pTYAa01, designed to generate a cdtABC knockout with a kanamycin-resistance cassette via homologous recombination. (B) pTYAa02, designed to generate a cdtABC knockout with a spectinomycin-resistance cassette. (C-E) pTYAa03 ( cdtA -His 6 ), pTYAa04 ( cdtB -His 6 ), and pTYAa05 ( cdtC -His 6 ), cloned with 5’UTRs including the native promoter region of the cdtABC operon. (F) pTYAa06, designed to generate a cdtABC complement with a spectinomycin-resistance cassette via homologous recombination.
Article Snippet: Approximately 1-kb fragments flanking the km cassette and the spectinomycin-resistance cassette (amplified from pUTmini-Tn5 Sm) were assembled into
Techniques: Knock-Out, Homologous Recombination, Clone Assay